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Ecologie larvaire de l'huître perlière (Pinctada margaritifera, L.), croissance et dispersion dans un lagon d'atoll polynésien ArchiMer
Thomas, Yoann.
Understanding key factors determining the bentho-pelagic species larval growth and dispersal remains one of the challenges of marine ecology, notably to understand the adult population structure and dynamics. In the present study, we explore this determinism on the development and dispersal of the black-lip pearl oyster (Pinctada margaritifera var. cumingii, Linné 1758) larvae in a French Polynesian atoll lagoon (Ahe, Tuamotu archipelago). Rearing of the pearl oyster for the production of black pearls takes on a major social and economic place in French Polynesia. However, the supply of pearl oyster seed to the farms depends entirely on the natural collection of wild spat of the only one specie P. margaritifera, and spatial and temporal variability of spat...
Tipo: Text Palavras-chave: Ecologie larvaire; Hétérogénéité environnementale; Modélisation; Croissance; Dispersion; Huître perlière à lèvres noires; Pinctada margaritifera; Lagon d’atoll; Polynésie française; Larval ecology; Environmental heterogeneity; Modeling; Growth; Dispersal; Black-lip pearl oyster; Pinctada margaritifera; Atoll lagoon; French Polynesia.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/00003/11449/7999.pdf
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Quantification of eggs and sperm in the Black-lip pearl oyster Pinctada margaritifera using an enzyme-linked immunosorbent assay (ELISA) ArchiMer
Jeung, Hee-do; Kang, Do-hyung; Park, Heung-sik; Le Moullac, Gilles; Choi, Kwang-sik.
We have developed immunological probes to quantify eggs and sperm of the Black-lip pearl (BLP) oyster Pinctada margaritifera. The western blot assay revealed that the polyclonal antibodies developed in this study specifically recognized only egg and sperm proteins. These polyclonal antibodies also showed high sensitivities to the antigens, detecting 0.31–10 μg/ml of the egg or sperm proteins in an indirect enzyme-linked immunosorbent assay (ELISA). Accordingly, we used an indirect ELISA to quantify the eggs and sperm in BLP oysters collected in December 2009 from Weno Island in Micronesia and in May 2010 from Tahiti. The gonad somatic index (GSI), a ratio of gonad weight to somatic tissue weight, of the females collected from Weno Island ranged from 3.6 to...
Tipo: Text Palavras-chave: Black-lip pearl oyster; ELISA; Pinctada margaritifera; Polyclonal antibody; Quantification of eggs and sperm.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00175/28659/32145.pdf
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